Molecular biology design for protein expression

Managing plasmids for expression

Transient expression of antibody and antigens in cell systems

Characterize purified antibodies in variety of ways (Protein or Cell binding)

Preform cell-based experiments to validate protein design concepts

Bachelors or Masters in biochemistry, molecular biology, or related field

Industry experience preferred.

Technical expertise in molecular biology with proficiency in generating constructs for production of antibody and bispecific molecules

Transient expression in HEK293 and CHO systems

Excellent sterile tissue culture technique for routine maintenance of primary and cancer cell lines and subsequent implementation in cell based assays

Good documentation, organization, and communication skills

Highly motivated with ability to work collaboratively in a team environment

Single color or multi-color flow cytometry

Functional protein characterization by ELISA or Octet

Primary cell isolation from blood

Data processing and analysis software (FlowJo, GraphPad Prism, Geneious)

Work collaboratively with other researchers to advance various therapeutic antibodies in different cancer types

Design, conduct, and interpret high quality ex vivo studies (e.g. ADCC, ADCP, cytokine profiling, gene expression profiling) and in vivo studies (e.g. syngeneic, xenograft, and/or humanized mouse models of cancer) to evaluate efficacy and mechanism of action (MOA) of Dren Bio’s novel therapeutic agents

Specific tasks include animal or human tissue processing, multi-color flow cytometry for immuno-phenotyping, cytokine profiling by MSD or Luminex, ADCC or ADCP assays using Incucyte live-cell imaging and flow cytometry, IHC/IF and confocal microscopy, DNA/RNA extraction for gene expression studies, various biochemical techniques (IP, western blot, IHC, and IF)

Develop and execute assays to evaluate antigen presentation by macrophages and dendritic cells, and T cell activation

Well-versed in various data processing and analysis software (e.g. FlowJo, Prism, ImageJ)

Mentor and train other scientists on novel tools and techniques

Become a project leader for a specific therapeutic program, while demonstrating flexibility and enthusiasm to support evolving company goals that are inherent of a fast-paced start-up environment

Meticulous record-keeping of scientific data, methods, and study designs

Present data in various forums, including lab meetings, department meetings, and multi-functional project teams

Publish scientific and methodological results in high quality journals

PhD in Immunology, Cancer Biology, Biochemistry, or related discipline with approximately 0-3+ years of experience in academia or biopharmaceutical industry.

The candidate is expected to have in-depth training in immunology and rich technical expertise in cellular and molecular immunology

Routine literature search and keeping up to date of the competitive landscape of immuno-oncology

Strong expertise in animal or human tissue processing and downstream assays (i.e. immuno-phenotyping by multi-color flow cytometry, immunohistochemistry, cell-based functional assays, biochemical assays)

Hands-on experience in designing and executing in vivo studies (e.g. syngeneic, xenograft, PDX, humanized mouse models of immuno-oncology)

Proficiency in IP, IV, SC and PO dosing, blood and tissue collection are required

Experience with primary cell isolation and culture

Must have a record of successful scientific accomplishments as evidenced by one or more first-author papers published or accepted in a well-recognized and peer-reviewed journals

Strong analytical and critical thinking skills with the ability to interpret complex data and highly motivated to learn and support the drug development efforts

Motivated self-starter who can work independently and collaboratively in a dynamic team environment with a proactive attitude

Excellent written and verbal communication skills

Ability to work in a fast-paced environment and the flexibility to align daily work with project needs

Ability to set goals and prioritize tasks and resources to achieve superior work quality and efficiency

Must be well organized, have a strong work ethic, attention to detail and be diligent in data documentation

As an integral member of the Protein Sciences group, this individual will work closely with other team members and partner with other functional areas in the organization

Production of proteins for Research and pre-clinical activities

Protein expression in mammalian system in transient and stable format

Protein purification using affinity and other chromatography methods

Cryopreservation of cell lines and clarification of production medium

Protein quantification, SDS-PAGE, size exclusion HPLC, other analytical methods as appropriate

Materials management and inventory maintenance

Maintenance of electronic lab notebook and databases, preparation of reports, and regular presentations to summarize results and update management

BS degree in biological sciences (biochemistry, cell biology, molecular biology) with 1-3 years experience in the biopharmaceutical industry

Familiarity with mammalian protein expression systems in transient and stable formats, protein purification of monoclonal antibody and other proteins using affinity and other chromatography techniques

Proficiency in aseptic technique, cell line maintenance, and host cell transfection

Experience with analytical methods such as SDS-PAGE, size exclusion HPLC, ELISA, Octet and other methods for protein characterization

Capacity for learning new techniques and handling diverse tasks concurrently

Ability to collaborate and communicate well with colleagues

Ability to work independently or with limited direct supervision

The ideal candidate will be meticulous, highly organized, pay great attention to detail, deliver consistent results, and work under aggressive timelines

The ideal candidate will have strong technical aptitude for lab and instrument work, and a problem-solving mindset that is diligent and flexible

Routinely run HPLC (SE-HPLC), capillary electrophoresis (Reduced and Non-reduced), icIEF, and ELISA based analytical methods for protein/ antibody characterization.

Help develop the above methods for all the molecules in Dren’s pipeline.

Work closely with colleagues in Process Sciences to ensure timely analytical support for cell line, process, and formulation development.

Facilitate the development of SOPs for various analytical methods and the qualification of methods required for technical transfer to CMO/CRO facilities.

Maintain and troubleshoot analytical instrumentation, as required.

Troubleshoot assay performance, as needed.

Document experimental work in electronic lab notebooks, author SOPs, reports.

Communicate results in group meetings.

BS degree in biological sciences (biochemistry, cell biology, molecular biology) with 0-6 months experience in the biopharmaceutical industry

Hands-on knowledge of technical scientific principles for most of the following analytical techniques: HPLC/UPLC (reverse phase, ion exchange, size exclusion), capillary electrophoresis (reduced and non-reduced CE-SDS), charge heterogeneity (icIEF), ELISAs

Strong analytical and critical thinking skills with the ability to interpret obtained results/data

Highly motivated to learn and support the drug development efforts at Dren Bio

Motivated self-starter who can work independently and collaboratively in a dynamic team environment

Excellent written and verbal skills

Ability to work in a fast-paced environment and the flexibility to align daily work with project needs

Must be well organized, have a strong work ethic with attention to details, and diligent in data documentation.